Figure 1.
In vitro cytotoxicity assay (MTS assay). (A) AdV5/3-D24-ICOSL-CD40L and AdV5/3-D24 at concentrations of 0.1, 1, and 10, and 100VP/cell were used to assess cell viability 96-h after infection. (B) Combinatory treatment with AdV5/3-D24-ICOSL-CD40L and AdV5/3-D24 at concentrations 100VP/cell with or without anti-PD-1 (100 µg/mL) were used to assess cell viability 96 h after infection. Statistical analyses were carried out with Mann–Whitney t-test. Error bars, mean ± SEM; **p ≤ 0.01, ***p<0.001.