Figure 1.

Flowchart of scCURE-based immunotherapy scRNA-seq data analysis

“scRNA-seq” demonstrates the data acquiring procedure. “scCURE” illustrates the model construction and discrimination of changed and unchanged cells. “Unchanged cells for prediction” illustrates the cell proportion characteristics are related to immunotherapy outcomes. In clinical application scenarios, an scCURE model can be constructed using training data and applied on testing patient to identify unchanged cells. “Changed cells for mechanisms interpretation” illustrates how to interpret immunotherapy mechanisms on changed cells.