Figure 3.

scCURE identified R-like and NR-like CD8⁺ T cells from pre-treatment patients with melanoma

(A) The AUC and p values of prediction models obtained using different choices of scCURE parameters.

(B) t-SNE plot of the scCURE-identified unchanged CD8⁺ T cells colored by cell type, cell group, and ICB response pattern.

(C) Heatmap of canonical CD8⁺ T cell functional markers.

(D) Chord diagram showing the association between cell clusters and R-like/NR-like categories by hypergeometric distribution test.

(E) Bar plot showing the well distribution of R-like/NR-like ratios between Rs and NRs.

(F) Signature scores of CD8-C2-SELL cluster computed on the PREJEB23709 dataset (Rs = 49, NRs = 42) and compared between cell groups with a two-sided Wilcoxon test. Signature scores were defined by the average expression of the top 30 CD8-C2-SELL markers. Statistical significance indicated by asterisks is as follows: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

(G) Hallmark pathways enriched in different CD8⁺ T cell clusters (Benjamini-Hochberg [BH]-adjusted p < 0.05).