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. 2023 Nov 20;3(11):100643. doi: 10.1016/j.crmeth.2023.100643

Figure 6.

Figure 6

Analyses of the changed macrophage cells in Rs show that antitumoral functions are activated by depolarization of M2 macrophages, which coordinates with the activation of CD8+ T cells

(A) Heterogeneity of the dynamical macrophage cells shown in the t-SNE scatterplot. Cell cluster frequency shown as a fraction of total cells in pre-treatment and post-treatment.

(B) Heatmap showing the signature scores for M1 and M2 macrophages.

(C) Enrichment of different cell clusters in pre- and post-samples.

(D) Pseudotime trajectory reconstruction and its association with cell clusters and sample labels.

(E) Enriched GSEA hallmarks of two representative macrophage clusters of pre- and post-treatment samples (Benjamini-Hochberg [BH]-adjusted p < 0.05).

(F) Survival analysis using the top 30 markers of Macro-C2-CCL18 and Macro-C3-CXCR4.

(G) Nichnet cell-cell communication between macrophages and CD8+ T cells in NRs reveals that the decrease in TNF and IFN-γ on CD8+ T cells is correlated with macrophage depolarization and proinflammatory function activation.