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Unimodal oligo distribution and cloning
(A) Example of unimodally distributed oligos in density plot after NGS.
(B) Example of 1 × 10 cm Petri dish with >4000 bacterial colonies.
(C) Schemes of cloning steps 10–21. In step 1, candidate region with KpnI and XbaI sites and barcode (BC) is cloned into ‘empty MPRA vector’ via SfiI sites, whilst in cloning step 2, KpnI and XbaI sites are used to insert CMV - GFP insert into the ordered oligo.
