| Reagent | Amount |
|---|---|
| 5× Q5 reaction buffer | 10 μL |
| Formamide (5%) | 2.5 μL |
| 10 μM dNTPs | 1 μL |
| Library Prep Forward Primer A (2 μM) | 2.5 μL |
| Library Prep Reverse Primer A (2 μM) | 2.5 μL |
| Cloning step 1 plasmid maxi prep | 50 ng |
| Q5 polymerase | 0.5 μL |
| ddH2O | To 50 μL |
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