| Step | Temperature | Time | Cycles |
|---|---|---|---|
| Initial denaturation | 98°C | 30 s | 1 |
| Denaturation | 98°C | 10 s | ×18 |
| Annealing | 55°C | 10 s | |
| Extension | 72°C | 10 s | |
| Final Extension | 72°C | 2 min | 1 |
| Hold | 10°C | forever | |
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