| Reagent | Amount |
|---|---|
| T4 DNA ligase buffer (10×) | 2 μL |
| Digested MPRA backbone | 100 ng |
| Digested CMV-GFP fragment | variable |
| T4 DNA ligase | 1 μL |
| ddH2O | To 20 μL |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.