Wnt antagonism-based chondrogenic medium

Reagent	Final concentration	Amount
L-glutamine	2 mM	5 mL
L-AA 2-P	0.1 mM	5 mL
ITS Liquid Media Supplement (100×)	1×	5 mL
Dexamethasone	0.1 μM	1 mL
Penicillin-Streptomycin (10,000 U/mL)	100 U/mL	5 mL
DMEM, low glucose, pyruvate	N/A	479 mL
XAV939	10 μM	0.5 mL
Total	N/A	500 mL

Store at 4°C and used within two weeks.