Table 1.

List of antibodies used to characterize the TIME (membrane staining)

Antibody	Clone	Fluorochrome	Concentration; dilution	Company and catalog #
Macrophages panel
CD11b	M1/70	PerCP-eFluor 710	0.2 mg/mL; 0.1 μL/100 μL	eBioscience # 46-0112-82
Ly6G/Ly6C (Gr)	RB6-8C5	eFluor 506	0.2 mg/mL; 0.1 μL/100 μL	eBioscience # 69-5931-82
F4/80	BM8	SB436	0.2 mg/mL; 0.8 μL/100 μL	eBioscience # 62-4801-82
CD206	MR6F3	PE	0.2 mg/mL; 0.1 μL/100 μL	eBioscience # 12-2061-82
MHC-II	M5/114.15.2	APC	0.2 mg/mL; 0.05 μL/100 μL	eBioscience # 17-5321-82
Lymphocytes T panel
CD4	RM4-5	FITC	0.5 mg/mL; 0.08 μL/100 μL	eBioscience # 11-0042-82
CD8	53–6.7	APC-eFluor 780	0.2 mg/mL; 0.42 μL/100 μL	eBioscience # 47-0081-82
Myeloid Derived Suppressor Cells panel
CD11b	M1/70	PerCP-eFluor 710	0.2 mg/mL; 0.05 μL/100 μL	eBioscience # 46-0112-82
Ly6C	HK1.4	APC-eFluor 780	0.2 mg/mL; 0.65 μL/100 μL	eBioscience # 47-5932-82
Ly6G	1A8-Ly6g	FTIC	0.5 mg/mL; 0.65 μL/100 μL	eBioscience # 11-9668-82
CD11c	N418	SB436	0.2 mg/mL; 0.65 μL/100 μL	eBioscience # 62-0114-82
Galectin 3	M3/38	eFluor 660	0.2 mg/mL; 0.25 μL/100 μL	eBioscience # 50-5301-82
Lymphocytes B panel
CD45	EM-05	PerCP	0.5 mg/mL; 0.2 μL/100 μL	eBioscience # MA1-10234
CD19	6D5	BV421	0.25 mg/mL; 1μL/100 μL	BioLegend # 115537
B220	RA3-6B2	Alexa Fluor 647	0.5 mg/mL; 0.1 μL/100 μL	BioLegend # 103226
CD38	90	APC-eFluor 780	0.2 mg/mL; 0.1 μL/100 μL	eBioscience # 47-0381-82
CD138	300506	PE	0.2 mg/mL; 0.2 μL/100 μL	eBioscience # MA5-23527