Cell culture media

Reagent	Final concentration	Amount
DMEM Dulbecco’s modified Eagle’s medium (DMEM), high glucose (4.5 g/L), L-glutamine (2 mM)	N/A	450 mL
FBS (Fetal Bovine Serum)	10%	50 mL
P/S (Penicillin/Streptomycin)	1%	5 mL
Total		555 mL

Note: Store at 4°C. Stability for more than 6 months. Pre-warm the culture medium at 37°C before use.