Figure 2.
The activity-based assay assesses HR deficiency in primary ovarian cancer cells
(A) Primary ovarian cancer cells were arranged in order of HR activity scores, representing high to low HR activity. The cells with HR activity higher than the HR deficiency (HRD) threshold, an HR activity score of 12 (dashed line), were classified as the HR-proficient (HRP) group and represented by a color palette ranging from green to cyan. Cells having HR activity ≤12 were classified as the HRD group and represented by a color palette ranging from brown to red-violet. The heatmaps for color palettes and associated HR activity scores are indicated at right. The primary ovarian cancer cells derived from an individual patient’s tumor tissue have been labeled in the form OCC-X, with respective ascites being labeled as OCC-X-a.
(B and C) Primary ovarian cancer cells were treated with olaparib for 4 days, followed by measurement of the IC50 of olaparib by alamarBlue cell viability assay. The dashed line represents 50% viability.
(B) The survival curves for HRP cells.
(C) The survival curves for HRD cells.
(D) HR activity and PARPi sensitivity of cancer cells derived from tumor tissue or ascites were analyzed separately and then subjected to Spearman correlation test, assessing the relationship between two variables.
∗∗∗∗p < 0.0001. Data are the mean ± SEM from three independent experiments (n = 3 biological replicates).
See also Figures S2 and S3.