REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-nucleoprotein monoclonal antibody blend (Light Diagnostics Rabies DFA Reagent)	EMD Millipore	5100
rabbit anti-Cre polyclonal antibody	Millipore Sigma	69050
Alexa Fluor 594-conjugated donkey anti-rabbit secondary	Jackson Immuno	711-585-152
chicken anti-GFP polyclonal antibody	Aves Labs	GFP-1020
Alexa Fluor 594-conjugated donkey anti-chicken secondary antibody	Jackson Immuno	703-585-155
Bacterial and virus strains
LV-TTBL(VSVG)	this paper	Addgene 115233 (genome plasmid)
RVΔG-4Cre(B19G)	Ref. Chatterjee et al.5	Addgene 98034 (genome plasmid)
RVΔG-4Flpo(B19G)	Ref. Jin et al.35	Addgene 122050 (genome plasmid)
RVΔGL-4Cre(B19G)	Ref. Chatterjee et al.5	Addgene 98039 (genome plasmid)
RVΔGL-4Flpo(B19G)	Ref. Chatterjee et al.5	Addgene 98040 (genome plasmid)
RVΔL-5Cre(B19G)	this paper	Addgene 182964 (genome plasmid)
RVΔL-5Flpo(B19G)	this paper	Addgene 182965 (genome plasmid)
RVΔL-5tTA(B19G)	this paper	Addgene 182966 (genome plasmid)
Chemicals, peptides, and recombinant proteins
DMEM	Thermo	11995073
antibiotic-antimycotic	Thermo	15240096
fetal bovine serum	VWR	16777–014
DPBS	Thermo	14190144
96-well 0.45um PVDF filter plates	Millipore	MSHVN4510
ProLong Diamond Antifade Mountant	Thermo	P36970
ProLong Diamond Antifade Mountant with DAPI	Thermo	P36971
Experimental models: Cell lines
BHK-21 [C13]	ATCC	BHK-21 [C13]
HEK 293T/17	ATCC	HEK 293T/17
BHK-B19G3_2	this paper	available upon request
BHK-B19L_2	this paper	available upon request
BHK-B19L-G_1	this paper	available upon request
293T-TREtight-EGFP	this paper	available upon request
293T-FLEX-F14F15S-BC	this paper	available upon request
293T-FLEX-F14F15S-BC-TTBL	this paper	available upon request
Experimental models: Organisms/strains
Ai14	Ref. Madisen et al.28	Jackson Laboratory 007914
Ai65F	Ref. Daigle et al.29	Jackson Laboratory 032864
Ai63	Ref. Daigle et al.29	gift from Hongkui Zeng, Allen Institute for Brain Science
Ai94D	Ref. Madisen et al.55	Jackson Laboratory 024104
ROSA:LNL:tTA	Ref. Wang et al.56	Jackson Laboratory 011008
Recombinant DNA
pLV-TTBL	Ref. Jin et al.4	Addgene 115233
pRVdG-4Cre	Ref. Chatterjee et al.5	Addgene 98034
pRVdG-4Flpo	Ref. Jin et al.35	Addgene 122050
pRVdGL-4Cre	Ref. Chatterjee et al.5	Addgene 98039
pRVdGL-4Flpo	Ref. Chatterjee et al.5	Addgene 98040
pRVdL-5Cre	this paper	Addgene 182964
pRVdL-5Flpo	this paper	Addgene 182965
pRVdL-5tTA	this paper	Addgene 182966
pB-TREtight-EGFP	this paper	Addgene 182967
pB-CAG-B19G-IRES-EGFP-WPRE-BGHpA	this paper	Addgene 178517
pB-CAG-FLEX-F14F15S-BFP-(mCherry)’-WPRE-BGHpA	this paper	Addgene 201831
Software and algorithms
Prism 9	GraphPad Software	Version 9.5.0 (525)
ImageJ	Ref. Schneider et al.57	https://ImageJ.nih.gov/ij/
FIJI	Ref. Schindelin et al.58	https://ImageJ.net/software/fiji/
pClamp 10	Molecular Devices	pClamp 10