Figure 2.

Discovery of Tn3 binders against human MALT1 and EED.A, progression of MALT1 (left) or EED (right) (50 nM tetramer) binding to two groups of yeast-displayed Tn3 libraries over four rounds of selection. Library group 1 consisted of four pooled loop libraries, and library group 2 consisted of two concave and one flat library (Fig. S3B). The percentage on the right-hand side of each plot represents the percentage of total yeast binding to the target protein. B and C, on-yeast MALT1 (B) or EED (C) binding titrations of individual clones isolated from Tn3 library selections, as measured by flow cytometry analysis. Signal was normalized to expression (detected with a V5 tag) for each Tn3 variant. EED, embryonic ectoderm development protein; MALT1, mucosa-associated lymphoid tissue lymphoma translocation protein 1; Tn3, third fibronectin type III domain of human tenascin-C.