REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Peroxidase AffiniPure goat anti-human IgG (H + L) antibody	Jackson ImmunoResearch	Cat# 109-035-003 RRID: AB_2337577
Bacterial and virus strains
VSV-G pseudotyped ΔG-luciferase	Kerafast	Cat# EH1020-PM
Biological samples
“4-dose monovalent” sera	This paper	N/A
“3-dose monovalent + bivalent” sera	This paper	N/A
“BA.5 breakthrough” sera	This paper	N/A
“BQ breakthrough” sera	This paper	N/A
Chemicals, peptides, and recombinant proteins
Polyethylenimine (PEI)	Polysciences Inc.	Cat# 23966-100
SARS-CoV-2 D614G S2P	Wang et al.13	N/A
SARS-CoV-2 BA.5 S2P	Wang et al.13	N/A
SARS-CoV-2 BQ.1.1 S2P	Wang et al.15	N/A
Critical commercial assays
Luciferase Assay System	Promega	Cat# E4550
QuikChange Lightning Site-Directed Mutagenesis Kit	Agilent	Cat# 210518
TMB substrate	Sigma-Aldrich	Cat# T0440
Experimental models: Cell lines
HEK293T	ATCC	Cat# CRL-3216; RRID: CVCL_0063
Vero-E6	ATCC	Cat# CRL-1586; RRID: CVCL_0574
Recombinant DNA
pCMV3-D614G	Wang et al.13	N/A
pCMV3-BA.2	Wang et al.13	N/A
pCMV3-BA.4/5	Wang et al.13	N/A
pCMV3-BQ.1.1	Wang et al.15	N/A
pCMV3-CH.1.1	Wang et al.16	N/A
pCMV3-XBB.1.5	This paper	N/A
pCMV3-XBB.1.16	This paper	N/A
pCMV3-XBB.1.5-E180V	This paper	N/A
pCMV3-XBB.1.5-K478R	This paper	N/A
Software and algorithms
GraphPad Prism 9	GraphPad Software Inc	https://www.graphpad.com/scientific-software/prism/