Figure 3.

Integrated TAM states highlight differences between AS and OD tumors

(A) UMAP embedding of integrated microglia from snRNA-seq data of primary OD and AS tumors, colored by assigned TAM subpopulations. UMAP1, x axis; UMAP2, y axis.

(B) Bar plots indicating TAM subpopulation proportions, separated by tumor subtype and grade.

(C) Boxplots showing TAM subpopulation proportions by tumor type. OD, oligodendroglioma; AS, astrocytoma. Wilcoxon rank-sum test was performed to test for significance.

(D) Representative IHC staining for TAM markers IBA1, CD74, and CD163 and double staining for p-STAT1 and IBA1 (60×) in OD and AS tumors. Images are captured at either 40× (scale bar, 50 μm) or 60× original magnification (scale bar, 30 μm)

(E) Plots showing semi-quantitative histological scores (0 = 0%, 1 = 0%–5%, 2 = 6%–29%, 3 = 30%–69%, 4 >70% positive staining) for p-STAT1⁺IBA1⁺ TAMs in OD and AS tumors, separated by grade (n = 3 per group).

(F and G) Select statistically significant receptor-ligand interactions between OPC-like and astro-like tumor populations (source) and the TAM subpopulations (target) (F) and TAM subpopulations (source) and OPC-like and astro-like tumor cells (target) (G). Dot size represents significance (adjusted p values); dot color reflects expression magnitude (means of average expression level of the interacting pair of genes).