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. 2023 Nov 1;4(11):101253. doi: 10.1016/j.xcrm.2023.101253

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© 2023 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Characterization of Sal4 IgA2_mRNA from in vitro transfection

(A) Binding of mRNA transfection supernatant and recombinant protein of Sal4 IgA2 to STm.

(B) Binding of SEC-separated Sal4 IgA2_R and supernatant from mRNA-transfected Sal4 IgA2 to immobilized human pIgR.

(C) Analytical SEC of affinity-purified IgA2 from mRNA transient transfection. Dimer (d) and monomer (mon) peaks are denoted. Transfections with JC are in blue and without JC are in orange, with aggregates (ag) labeled.

(D) SDS-PAGE of designated SEC peaks from mRNA transient transfection under reducing (R) and non-reducing (NR) conditions. dimer and monomer for NR are denoted along with HCs, LCs, and JCs in R conditions.

(E) Representative image of negative-stain EM 2D class averages of the dimeric peak isolated from mRNA transfection with J chain.

(F) Effect of IgA2_R or IgA2_mRNA on STm invasion of HeLa cells. Each symbol represents a replicate, and data is graphed mean ± SD of 3 replicates, ∗∗p < 0.05.