Figure 3.
IgA2mRNA expresses in serum, traffics to mucosa, and can block STm invasion into mouse Peyer’s patches
(A–C) Adult BALB/c mice were injected intravenously with 1 mg/kg formulated antibody encoded by mRNA modified with 3′idT, 2.5 mg/kg IgG1R, or 2.5 mg/kg dIgA2R. Concentrations of antibody were measured in (A) serum and (B) feces over time by isotype-specific ELISA.
(C) Comparison of serum and feces levels at 24 h following administration in mice receiving different test articles.
(D) STm challenge outlined for (E)–(H) where adult BALB/c mice were injected intravenously with 1 mg/kg formulated antibody encoded by mRNA modified with 3′idT, 5 mg/kg recombinant antibody protein, or saline. Mice were challenged 24 h later with an oral gavage of a one-to-one mixture of O5+ and O5− strains (4 × 107 colony-forming units [CFUs] total).
(E and F) Concentrations of antibody were measured in (E) serum and (F) feces prior to challenge by isotype-specific ELISA.
(G) Comparison of serum and fecal antibody levels immediately prior to challenge.
(H) Competitive indices of O5+ and O5− STm. Dashed line represents the limit of detection of the assay.
Each group in (A)–(C) represents the mean ± SD of 4–8 mice per group. Each symbol in (D)–(G) represents an individual mouse with mean ± SD depicted. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.