Fig. 3.

Enhanced 2′-FL production by enhancing GDP-d-mannose synthesis pathway. (A) Schematic view of the strategies to strengthen GDP-d-mannose synthesis by co-overexpressing of PMI40, SEC53, and PSA1. (B) 2′-FL production of the strains F3-5 and the strain F4-2. (C) 2′-FL yield on glucose and 2′-FL titer (g/g DCW) of the strain F3-5 and the strain F4-2. The data was derived from the results of three repeated experiments. T-test was performed to determine p values. ***p < 0.001; ****p < 0.0001.