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. 2023 Nov 15;8(4):716–723. doi: 10.1016/j.synbio.2023.11.002

Fig. 4.

Fig. 4

The enhancement of NADPH regeneration pathway promotes 2′-FL accumulation. (A) Schematic view of the enhanced NADPH regeneration pathway. (B) 2′-FL production and 2′-FL yield on glucose of the strains overexpressing ZWF1, PGL, and GND1, respectively. (C) 2′-FL titer of the strain F4-2 and the strain F5-1. (D) 2′-FL yield on glucose and OD600 of the strain F4-2 and the strain F5-1. The data was derived from the results of three repeated experiments. T-test was performed to determine p values. **p < 0.01; ***p < 0.001; ****p < 0.0001.