Figure 1.

PDOs established from BTC patients

(A) Experimental design. Fresh BTC samples were obtained from patients (patient information is detailed in Table S1) and processed as described in STAR Methods.

(B) Pie charts comparing the stratification of all tumors with that of all derived organoids based on histological type (top) and tumor-node-metastasis (TNM) stage (bottom).

(C) Representative H&E and bright-field microscopy images of patient-derived BTC organoids and matched primary tumors from three main histological subtypes. Scale bars, 50 μm.

(D) IHC analysis for the BTC markers (CK19 and CK7) on parental tumors and organoids. Scale bars, 50 μm.