Figure 3.

Clinically relevant responses to chemotherapy in BTC organoids ex vivo

(A) The heatmap consists of 47 BTC organoids’ chemosensitivity responses, which included gemcitabine, cisplatin, 5-FU, SN-38, oxaliplatin, mitomycin C, and paclitaxel (n = 3). Chemosensitivity responses were awarded by AUC Z scores. Colors range from blue (low scores, meaning resistant) to red (high sores, meaning sensitive).

(B) Summary of chemosensitivity responses for 47 BTC organoids ex vivo to gemcitabine, cisplatin, 5-FU, and oxaliplatin, and results are displayed in the form of dose-response curves (n = 3). AUC was calculated from the raw dose-response data and is displayed as a violin plot.

(C) Effects on the viability of chemotherapy drug using an organoid-formation assay. Red square, resistant; no square, no viable cells. Scale bars, 100 μm.