| Chemicals, peptides, and recombinant proteins |
|
| Calcofluor white stain |
Sigma-Aldrich |
18909 |
| Fetal bovine serum |
Serana |
FBS-AU-015 |
| Penicillin-Streptomycin |
Sigma-Aldrich |
P4333 |
| Peptone |
US Biological |
73049-73-7 |
| Yeast extract |
US Biological |
8013-01-2 |
| Uridine |
Sigma-Aldrich |
58-96-8 |
| CellTracker Green CMFDA dye |
Thermo Fisher Scientific |
C7025 |
| DRAQ7 dye |
Abcam |
ab109202 |
|
| Experimental models: Organisms/strains |
|
|
C. albicans: CAF2.1-dTom-NATr: URA3/ura3::λimm434, IRO1/iro1::λimm434, ENO1/eno1::dTom-SAT1 |
Lionakis et al.17
|
YCAT1127 |
| Mouse: wild-type C57BL/6J, male or female, aged 6–8 weeks |
Monash Animal Research Platform (Melbourne, Australia) |
RRID: IMSR_JAX:000664 |
|
| Software and algorithms |
|
| Bio-Formats |
24 |
The Open Microscopy Environment: https://www.openmicroscopy.org/bio-formats/
|
| CellProfiler 2.1.1 |
McQuin et al.16
|
CellProfiler: https://cellprofiler.org/previous-releases
|
| ImageJ 2.0.0-rc-69 |
Schindelin et al.25
|
ImageJ: https://imagej.net/ij/download.html
|
| GraphPad Prism 9.0.0 |
GraphPad Software, San Diego, California, USA |
GraphPad: https://www.graphpad.com/features
|