REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
β-Actin (8H10D10) Mouse mAb	Cell Signaling Technology	Cat #3700; RRID:AB_2242334
p44/42 MAPK (Erk1/2) (3A7) Mouse mAb	Cell Signaling Technology	#9107; RRID:AB_10695739
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody	Cell Signaling Technology	#9101; RRID:AB_331646
Akt Antibody	Cell Signaling Technology	#9272; RRID:AB_329827
Phospho-Akt (Thr308) Antibody	Cell Signaling Technology	#9275; RRID:AB_329828
Stat5 (D2O6Y) Rabbit mAb	Cell Signaling Technology	#94205; RRID:AB_2737403
Phospho-Stat5 (Tyr694) Antibody	Cell Signaling Technology	#9351; RRID:AB_2315225
Bcl-2 (124) Mouse mAb	Cell Signaling Technology	#15071; RRID:AB_2744528
Bcl-xL Antibody	Cell Signaling Technology	#2762; RRID:AB_10694844
Mcl-1 Antibody	Cell Signaling Technology	#4572; RRID:AB_2281980
Bim (C34C5) Rabbit mAb	Cell Signaling Technology	#2933; RRID:AB_1030947
BMF (E5U2J) Rabbit mAb	Cell Signaling Technology	#50542; RRID:AB_2892182
IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody	LI-COR Biosciences	Cat# 925-32211 RRID:AB_2651127
IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody	LI-COR Biosciences	Cat# 925-68070, RRID:AB_2651128
Bmf Antibody	Novus Biologicals	Cat# NBP1-76658, RRID:AB_11040482
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647®	Thermo Fisher Scientific	Cat# A-21244, RRID:AB_2535812
Alexa Fluor® 647 anti-Cytochrome c Antibody	BioLegend	Cat# 612310, RRID:AB_2565241
PE anti-mouse CD45 Antibody	BioLegend	Cat# 103105, RRID:AB_312970
APC/Cyanine7 anti-human CD45 Antibody	BioLegend	Cat# 304014, RRID:AB_31440
CD45 (Intracellular Domain) (D9M8I) XP® Rabbit mAb	Cell Signaling Technology	Cat# 13917, RRID:AB_2750898
Bacterial and virus strains
pCW57.1		RRID:Addgene_41393
NEB 10-beta Competent E. coli (High Efficiency)	New England Biolabs	Cat# C3019I
Biological samples
PDX #1	This paper	HM0007
PDX #2	Qin et al.61	CD33-CART-0005
AML patient and healthy donor samples	UCSF Hematologic Malignancies Tissue Bank
Chemicals, peptides, and recombinant proteins
RMC-4550	Revolution Medicines	ID: 0694550-018
Venetoclax (ABT-199)	Selleckchem	Cat# S8048; CAS No. 1257044-40-8
Gilteritinib (ASP2215)	Selleckchem	Cat# S7754; CAS No. 1254053-43-4
WEHI-539 HCl	Selleckchem	Cat# S7100 CAS No. 2070018-33-4
Trametinib (GSK1120212)	Selleckchem	Cat# S2673; CAS No. 871700-17-3
S63845	MedChem Express	Cat# HY-100741 CAS No. : 1799633-27-4
BIM peptide	New England Peptides	Cat# LB4609
Alamethicin	Enzo Life Sciences	Cat BML-A150-0005; CAS No. 27061-78-5
Digitonin	Milipore Sigma	Cat# D5628; Cas No. CAS Number: 11024-24-1
D-Mannitol	Milipore Sigma	Cat# M9647; CAS No. 69-65-8
Succinic acid	Milipore Sigma	Cat# S3674; CAS No. 110-15-6
Ethylenediaminetetraacetic acid	Milipore Sigma	Cat# E6758; CAS No. 60-00-4
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid	Milipore Sigma	Cat# E3889; CAS No. 67-42-5
HEPES Buffer, N-(2-Hydroxyethyl)piperazineN′-(2-ethanesulfonic acid)	Milipore Sigma	Cat# SRE0065;
Glycine	Thermo Fisher Scientific	Cat# BP381-1 CAS No. 56-40-6
Doxycycline Hydrochloride	Milipore Sigma	Cat# D3072 CAS No. 10592-13-9
Recombinant Human GM-CSF	PeproTech	Cat# 300-03; Genpept: P04141
Recombinant Human Flt3-Ligand	PeproTech	Cat# 300-19; Genpept: P49771
Recombinant Human FGF-basic	PeproTech	Cat# 100-18B; Genpept: P09038
Lipofectamine™ 3000	Thermo Fisher Scientific	Cat# L3000008
Lenti-X™ concentrator	TakaraBio	Cat# 631232
Triton™ X-100 Surfact-Amps™ Detergent Solution	Thermo Fisher Scientific	Cat# 85111
Paraformaldehyde Aqueous Solution	Electron Microscopy Sciences	Cat#15710-S
Normal Goat Serum (10%)	Thermo Fisher Scientific	Cat# 50197Z
ProLong™ Gold Antifade Mountant with DNA Stain DAPI	Thermo Fisher Scientific	Cat# P36935
D-Luciferin, Potassium Salt	Gold Biotechnology	Cat# LUCK-100; CAS No. 115144-35-9
Hematoxylin Instant	Thermo Fisher Scientific	Cat# 6765015
Critical commercial assays
CellTiter-Glo® Luminescent Cell Viability Assay	Promega	Cat# G7573
Pierce™ BCA Protein Assay Kits	Thermo Fisher Scientific	Cat# 23225
Ras G-LISA Activation Assay Kit (Colorimetric Based) - 96 Assays	Cytoskeleton	Cat# BK131
CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit	Thermo Fisher Scientific	Cat# C10427
RNeasy Mini Kit (50)	Qiagen	Cat# 74104
Discovery Purple Kit	Roche Diagnostics	Cat# 760229
Deposited data
CRISPR (DepMap Public, Chronos)	the Cancer Dependency Map	https://depmap.org
RNA-seq data	This paper	GEO: GSE217359
Experimental models: Cell lines
Molm-14	ATCC	RRID:CVCL_7916
MV4-11	ATCC	RRID:CVCL_0064
Kasumi-1	ATCC	RRID:CVCL_0589
SKNO-1	ATCC	RRID:CVCL_2196
OCIAML-3	ATCC	RRID:CVCL_1844
NOMO-1	ATCC	RRID:CVCL_1609
HL-60	ATCC	RRID:CVCL_0002
THP-1	ATCC	RRID:CVCL_0006
K562	ATCC	RRID:CVCL_0004
HEL	ATCC	RRID:CVCL_0001
U-937	ATCC	RRID:CVCL_0007
HS-5	ATCC	RRID:CVCL_3720
LentiX 293T	Takara Bio	RRID:CVCL_4401
Molm-14 PTPN11 WT-dox	This paper	N/A
Molm-14 PTPN11 E76K-dox	This paper	N/A
Molm-14 PTPN11 G60V-dox	This paper	N/A
Molm-14 PTPN11 S502P-dox	This paper	N/A
Molm-14 PTPN11 T253M/Q257L-dox	This paper	N/A
Molm-14 MEK DD-dox	This paper	N/A
Molm-14 AKT E17K-dox	This paper	N/A
MV4-11 MEK DD-dox	This paper	N/A
MV4-11 AKT E17K-dox	This paper	N/A
Molm-14 BMF KO	This paper	N/A
SKNO-1 BMF KO	This paper	N/A
Molm-14 FFLuc	This paper	N/A
Molm-14 NRAS G12C FFLuc	This paper	N/A
Kasumi-1 FFLuc	This paper	N/A
Molm-14 NRAS G12C	McMahon et al.14	N/A
Molm-14 NRAS Q61K	McMahon et al.14	N/A
Molm-14 FLT3 D835H	Smith et al.77	N/A
Molm-14 FLT3 D835V	Smith et al.77	N/A
Molm-14 FLT3 D835Y	Smith et al.77	N/A
Molm-14 FLT3 N841K	Smith et al.77	N/A
Molm-14 FLT3 Y842C	Smith et al.77	N/A
Molm-14 FLT3 F691L	Smith et al.77	N/A
Experimental models: Organisms/strains
NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ	The Jackson Laboratory	Strain #:005557 RRID:IMSR_JAX:005557
NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ	The Jackson Laboratory	Strain #:013062 RRID:IMSR_JAX:013062
Oligonucleotides
Human GAPDH Taqman probe	Thermo Fisher Scientific	Hs02758991_g1
Human OASL Taqman probe	Thermo Fisher Scientific	Hs00984387_m1
Human OAS1 Taqman probe	Thermo Fisher Scientific	Hs00973637_m1
Human IFIT2 Taqman probe	Thermo Fisher Scientific	Hs01922738_s1
Human BMF Taqman probe	Thermo Fisher Scientific	Hs00372937_m1
BMF sgRNA guide1: 5’-AAGGCCAGGGCCACAGCAGU-3’	Synthego	Custom ID
BMF sgRNA guide2: 5’- AAGCUCCCGGGUUGGGUCAC-3’	Synthego	Custom ID
BMF sgRNA guide1: 5’- GGAGCCAUCUCAGUGUGUGG-3’	Synthego	Custom ID
Software and algorithms
GraphPad Prism	http://www.graphpad.com/	RRID:SCR_002798
FlowJo	https://www.flowjo.com/solutions/flowjo	RRID:SCR_008520
R Project for Statistical Computing	http://www.r-project.org/	RRID:SCR_001905
STAR	Dobin et al.78	RRID:SCR_004463
Limma-voom package	Law et al.79
ShinyGO	http://bioinformatics.sdstate.edu/go/	RRID:SCR_019213
Gene Set Enrichment Analysis	Subramanian et al.39	RRID:SCR_003199
NIS Elements		RRID:SCR_014329
ImageJ	https://imagej.net/	RRID:SCR_003070
SynergyFinder 3.0	Ianevski et al.55
ICE	Conant et al.80