Fig. 5.

ARB regulates intracellular lipid metabolism and ferroptosis and promotes methylation modification of SLC7A11 by inhibiting FTO. (A) The effect of FTO and FTO-mut overexpression on intracellular TG content (n = 3–4 per group). (B–C) Oil red O staining and lipid droplet size analysis. (D) Effects of FTO overexpression on TC content (n = 3–4 per group). (E–F) ROS staining with MitoSOX red probe (n = 3–4 per group). (G–H) Effect of FTO overexpression on intracellular GSH and SOD level (n = 3 per group). (I–K) Intracellular Fe, Fe²⁺ and Fe²⁺ to Fe³⁺ ratio in FTO overexpressing cells (n = 3 per group). (L–M) Calcein staining (n = 3 per group). (N) m6A dot blotting. (O) qPT-PCR of ferroptosis-related genes after FTO overexpression (n = 3 per group). (P–Q) Western Blot detects the effect of FTO overexpression on SLC7A11 protein levels (n = 3 per group). (R–S) Effect of ARB, FTO, and FTO-mut treatments on SLC7A11 mRNA half-life (n = 3 per group). (T–U) Merip-seq as well as Merip-qRCR (X: n = 3–4 per group). Data are mean ± SEM, n ≥ 3; One-way ANOVA was used to compare the means of three groups, ∗P < 0.05; ∗∗P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)