Fig. 1. 1H-NMR spectra of rutin (2) and the acylation reaction with palmitic acid in acetone-d6, yielding rutin palmitate (3d). Expansion 3.30–7.90 ppm: marked in rectangles are the shifts between the aromatic protons of rutin (5′ and 8) when the acyl ester is formed. Marked in the solid circle is the deshielded anomeric proton of glucose (1′′) and in the dashed circle is the deshielded anomeric proton of rhamnose (1′′′). Finally, the dashed squares include the shifts for proton 4′′′, which gets significantly deshielded when the ester is formed.
