Trials of rutin acylation with 40 kU L−1CalB immobilized on acrylic resin; 72 h, 55 °C, no mechanical stirring.
| Exp. no. | Solvent | Dehydrating agent | Fatty acid | Conversion ratio |
|---|---|---|---|---|
| 27 | 10 : 1 acetonitrile : DMSO | MS4Å (100 g dm−3) | Octanoic acid (10 eq.) | 16% |
| 28 | 10 : 1 acetonitrile : DMSO | MS4Å (100 g dm−3) | Palmitic acid (50 eq.) | 15% |
| 29 | 10 : 1 acetonitrile : DMSO | SiO2 (32.9 g dm−3) | Octanoic acid (10 eq.) | 20% |
| 30 | 10 : 1 acetonitrile : DMSO | SiO2 (32.9 g dm−3) | Octanoic acid (100 eq.) | 38%a |
| 31 | 10 : 1 acetonitrile : DMSO | SiO2 (32.9 g dm−3) | Octanoic acid (100 eq.) | 45%b |
| 32 | 10 : 1 2-butanone : DMSO | SiO2 (32.9 g dm−3) | Octanoic acid (100 eq.) | 24% |
| 33 | 10 : 1 acetonitrile : DMSO | SiO2 (32.9 g dm−3) | Acetic acid (100 eq.) | Tracesc |
| 34 | 10 : 1 acetonitrile : DMSO | SiO2 (32.9 g dm−3) | Butyric acid (100 eq.) | 30% |
| 35 | 10 : 1 acetonitrile : DMSO | SiO2 (32.9 g dm−3) | 2-Methylbutyric acid (100 eq.) | 7% |
| 36 | 10 : 1 acetonitrile : DMSO | SiO2 (32.9 g dm−3) | Palmitic acid (50 eq.) | 19% |
| 37 | 10 : 1 acetonitrile : DMSO | SiO2 (32.9 g dm−3) | Stearic acid (50 eq.) | 8% |
| 38 | 10 : 1 acetonitrile : DMSO | SiO2 (32.9 g dm−3) | Oleic acid (50 eq.) | 15% |
| 39 | 10 : 1 acetonitrile : DMSO | SiO2 (32.9 g dm−3) | Methyl oleate (50 eq.) | 15% |
| 40 | 10 : 1 acetonitrile : DMSO | MS4Å (100 g dm−3) | p-Coumaric acid (10 eq.) | Nil |
| 41 | 10 : 1 acetonitrile : DMSO | SiO2 (32.9 g dm−3) | Lauric acid (50 eq.) | 30% |
a
Total reaction volume was 5 mL.
b
100 kU L−1CalB on acrylic resin.
c
Evidence of non-selective acylation.