TABLE 1.
List of SPD-CD40L fusions generated for this publication.
Construct | N-terminus* | Collagen | Coiled coil* | GS linker |
---|---|---|---|---|
Full-length SPD | Yes ** | 59 repeats (46–222) | Yes ** | No |
12R Col | Yes | 12 repeats (79–114) | Yes | No |
12R Col linker | Yes | 12 repeats (79–114) | Yes | (GGGS)x3 |
0R Col | Yes | No | Yes | No |
0R Col linker | Yes | No | Yes | (GGGS)x3 |
6R Col NGly | Yes | 12 repeats (79–96) | Yes | No |
6R Col | Yes | 12 repeats (97–114) | Yes | No |
12bisR Col | Yes | 12 repeats (121–156) | Yes | No |
19R Col | Yes | 19 repeats (79–135) | Yes | No |
30R Col | Yes | 30 repeats (46–135) | Yes | No |
*All constructs contained the same human SPD N-terminus and coiled coil and CD40L ectodomain at their C-terminus.
**Numbering according to human SPD (P35247): N-terminus (peptide 22–45) and coiled coil (peptide 223–252).