Figure 2—

Mean ± SE results for serotype transduction in tissue explants after vector inoculation and in uninoculated control tissue explants. A—In cartilage tissue explants (gray bars) at 6 days after vector inoculation at 3.0 X 10² GCs/cell, serotype rAAV2/6.2 had a significantly (P = 0.040) higher BPC, compared with rAAV2/2 and all other serotypes tested. Serotype rAAV2/6.2 also had a significantly (P < 0.001) higher BPC in synovial tissue explants (black bars) at 6 days after inoculation, compared with that for serotypes rAAV2/5 and rAAV2/2. B—Cartilage tissue explants at 6 (white bars), 30 (gray bars), and 60 days (black bars) after vector inoculation at 3.0 X 10² GCs/cell. At day 30, serotype rAAV2/2 had the highest BPC, followed by serotypes rAAV2/5 and rAAV2/6.2, whereas at day 60, serotype rAAV2/2 had the highest BPC, followed by serotypes rAAV2/5 and rAAV2/6.2. C—Synovial tissue explants at 6 (white bars), 30 (gray bars), and 60 days (black bars) after vector inoculation. Notice that at 30 and 60 days, serotype rAAV2/5 had the highest BPC, followed by serotype rAAV2/2. D—All vector serotypes, except for serotype rAAV2/6.2, had an increase in BPC from day 6 to day 30 after inoculation in cartilage tissue explants (gray bars) and synovial tissue explants (black bars). See Figure 1 for remainder of key.