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. 2023 Dec 4;21:877. doi: 10.1186/s12967-023-04760-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Effects of circKIF18B_003 on PCa Cell Proliferation, Invasion, and Migration. A The relative expression levels of circKIF18B_003 in DU145 and PC-3 cell lines post-transfection. DU145 cells were transfected with two shRNAs targeting circKIF18B_003 (for knockdown), while PC-3 cells were transfected with a plasmid promoting circKIF18B_003 overexpression. The graph shows a marked decrease in circKIF18B_003 expression in the DU145 cell line and a significant increase in the PC-3 cell line. B Colony formation assay results comparing the effects of circKIF18B_003 downregulation and upregulation on PCa cell viability. Downregulation of circKIF18B_003 led to a decrease in colony formation (indicating reduced cell viability), while upregulation had the opposite effect. C KIF18B protein expression levels in DU145 and PC-3 cells after transfection, showing that changes in circKIF18B_003 levels did not influence KIF18B expression. D Results from the Transwell invasion assay, demonstrating the impact of circKIF18B_003 modulation on PCa cell invasion capabilities. CircKIF18B_003 knockdown reduced cell invasion, while overexpression led to increased cell invasion. E CCK-8 assay results, further demonstrating the impact of circKIF18B_003 modulation on PCa cell viability. Consistent with the colony formation assay, circKIF18B_003 downregulation led to decreased cell viability, while its upregulation promoted cell viability. F Results from the wound-healing migration assay, illustrating the influence of circKIF18B_003 on PCa cell migration. Reduced cell migration was observed following circKIF18B_003 knockdown, while overexpression resulted in enhanced cell migration. Unpaired student’s test, Mann–Whitney U test, Kruskal–Wallis test and one-way ANOVA test were used for the statistical analyses. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001