. 2023 Dec 4;16:448. doi: 10.1186/s13071-023-06016-4

Table 1.

Primers used to amplify Borrelia genes in this study

Gene	Round	Primer name	Secuencia 5′–3′	Tm [°C]	bp
16S rRNA qPCR	Screening	Bor16S3F	AGCCTTTAAAGCTTCGCTTGTAG	60	148
		Bor16S3R	GCCTCCCGTAGGAGTCTGG
		probe Bor16S3P	[6FAM] CCGGCCTGAGAGGGTGAACGG
16S rRNA	First round	FD3 [f]	AGAGTTTGATCCTGGCTTAG	54	1489
	First round	T50 [r]	GTTACGACTTCACCCTCCT	54	1489
	Second round	FD3 [f]	AGAGTTTGATCCTGGCTTAG	56	730
	Second round	16 s-1 [r]	TAGAAGTTCGCCTTCGCCTCTG	56	730
	Second round	16 s-2 [f]	TACAGGTGCTGCATGGTTGTCG	56	462
	Second round	T50 [r]	GTTACGACTTCACCCTCCT	56	462
	Second round	Rec4 [f]	ATGCTAGAAACTGCATGA	54	520
	Second round	Rec9 [r]	TCGTCTGAGTCCCCATCT	54	520
flaB (flagellin)	First round	FlaRL [f]	GCAATCATAGCCATTGCAGATTGT	55	665
	First round	FlaLL [r]	ACATATTCAGATGCAGACAGAGGT	55	665
	Second round	FLaRS [f]	CTTTGATCACTTATCATTCTAATAGC	55	491
	Second round	FlaLL [r]	ACATATTCAGATGCAGACAGAGGT	55	491
	Second round	FlaRL [f]	GCAATCATAGCCATTGCAGATTGT	55	528
	Second round	FLaLS [r]	AACAGCTGAAGAGCTTGGAATG	55	528