Fig. 3.

LMP7/PSMB8 is essential for KMT2A-r AML development but dispensable for normal hematopoiesis. A Dot plots depicting % of GFP⁺ cells in peripheral blood of recipient mice transplanted with 7 × 10⁴ KMT2A::MLLT3 transformed LSKs from LMP7^+/+ (n = 12) or LMP7^−/− (n = 12) mice over 14 weeks. Two independent cohorts. B Kaplan–Meier survival curves of recipient mice (KMT2A::MLLT3 transformed LSKs from LMP7^+/+ (n = 12) or LMP7 ^−/− (n = 12) mice). Two independent cohorts; Mantel-Cox test. C White blood counts (WBC), hemoglobin (HGB) and platelets (PLT) in the peripheral blood of LMP7^−/− (n = 7) for 16 weeks of steady-state hematopoiesis, compared with LMP7^+/+ controls (n = 7). D Immunophenotypic quantification of progenitor cell abundance (Prog: Lin⁻ Sca1⁻ c-Kit⁺), common myeloid progenitors (CMP: Lin⁻ Sca1⁻ c-Kit⁺ CD34⁺ FcgR⁻), granulocyte–macrophage progenitors (GMP: Lin⁻ Sca1⁻ c-Kit⁺ CD34⁺ FcgR⁺) and megakaryocyte-erythroid progenitors (MEP: Lin⁻ Sca1⁻ c-Kit⁺ CD34⁻ FcgR⁻) in the bone marrow. E Peripheral blood chimerism over 16 weeks; competitive repopulation assay using BM cells from LMP7^−/− (n = 8) or LMP7^+/+ (n = 8) donors