Table 1.
Transcription rates of metal transporters and metal-related genesa
| Hfx. alexandrinus DSM 27206 ORFb | Gene name | Log2FC | Annotation | ||
|---|---|---|---|---|---|
| 0.1 mM | 0.25 mM | 0.5 mM | |||
| Hfx-2019-SC1-cds4 | copA | 3.25 | 4.48 | 5.64 | Cu+-exporting ATPase |
| Hfx-2019-SC1-cds5 | lrp | - | 2.2 | 3.73 | Transcriptional regulator |
| Hfx-2019-SC1-cds6 | copZ | - | - | 3.1 | Copper chaperone |
| Hfx-2019-SC1-cds149 | petE | 5.49 | - | - | Plastocyanin/azurin family |
| Hfx-2019-SC1-cds430 | 3.14 | - | - | HTH_ARSR domain containing protein | |
| Hfx-2019-SC3-cds305 | - | - | 2.09 | HTH_ARSR domain containing protein | |
| Hfx-2019-SC4-cds254 | 2.08 | - | 2.67 | ABC.PE.A1 peptide/nickel transport system ATP-binding protein | |
| Hfx-2019-SC4-cds255 | - | - | 2.84 | ABC.PE.A peptide/nickel transport system ATP-binding protein | |
| Hfx-2019-SC4-cds257 | - | - | 2.84 | ABC.PE.P peptide/nickel transport system permease protein | |
| Hfx-2019-SC4-cds258 | ddpA | - | - | 2.19 | ABC.PE.S peptide/nickel transport system substrate-binding protein |
| Hfx-2019-SC4-cds326 | petE | - | - | 3.06 | Plastocyanin/azurin family |
| Hfx-2019-SC5-cds117 | mntH | - | - | 2.43 | Manganese transport protein |
| Hfx-2019-SC4-cds164 | mobA | - | - | -5.72 | Molybdopterin-guanine dinucleotide biosynthesis protein A |
| Hfx-2019-SC4-cds165 | wtpA | - | - | -5.94 | Molybdate/tungstate transport system substrate-binding protein |
| Hfx-2019-SC4-cds166 | wtpB | - | - | -5.15 | Molybdate/tungstate transport system permease protein |
| Hfx-2019-SC4-cds220 | - | -2.68 | - | LucA/LucC family protein member | |
| Hfx-2019-SC4-cds224 | fepB | -2.17 | - | - | ABC.FEV.S iron complex transport system substrate-binding protein |
| Hfx-2019-SC4-cds239 | cibT | -2.16 | - | - | Cobalt-precorrin-6B (C15)-methyltransferase |
| Hfx-2019-SC4-cds240 | cbtB | -2.9 | - | - | Cobalt transporter subunit, putative |
| Hfx-2019-SC4-cds241 | cbtA | -2.83 | - | - | Cobalt transporter subunit, putative |
| Hfx-2019-SC4-cds243 | asnC | -2.09 | - | - | Lrp/AsnC family transcriptional regulator |
| Hfx-2019-SC4-cds337 | -2 | - | - | ABC.PE.P1 peptide/nickel transport system permease protein | |
aRNA-Seq was used to determine the expression levels of the genes in Hfx. alexandrinus DSM 27206, as detailed in the section on Materials and Methods. The data are the average of three replicates. The threshold of differential expression genes was: |log2(FoldChange)|> 1 and qvalue < 0.00. Dashes indicate that there were minimal changes to the transcription, not considered statistically significant. bThe gene name, number, and annotation were generated through the gDNA sequencing of the tested strain, as described in the Materials and Methods section