Table 2.
Transcription rates of genes involved in the oxidative stress response and energy metabolisma
| Hfx. alexandrinus DSM 27206 ORFb | Gene name | Log2FC | Annotation | ||
|---|---|---|---|---|---|
| 0.1 mM | 0.25 mM | 0.5 mM | |||
| Hfx-2019-SC1-cds370 | nhaC | 4.16 | 2.1 | 2.31 | Na+:H+antiporter |
| Hfx-2019-SC1-cds423 | norB | 2.35 | - | - | Nitric oxide reductase subunit B |
| Hfx-2019-SC3-cds277 | cydB | - | - | 2.61 | Cytochrome d ubiquinol oxidase subunit II |
| Hfx-2019-SC2-cds328 | nuoCD | 2.09 | - | 2.08 | NADH-quinone oxidoreductase subunit C/D |
| Hfx-2019-SC5-cds137 | 2.25 | - | 2.07 | Flavin-dependent oxidoreductase | |
| Hfx-2019-SC5-cds143 | putA | 3.11 | - | 2.77 | Aldehyde dehydrogenase (NAD+) |
| Hfx-2019-SC2-cds705 | porB | -2.09 | - | - | Pyruvate ferredoxin oxidoreductase beta subunit |
| Hfx-2019-SC2-cds772 | -2.34 | COG2210 Peroxiredoxin family protein member | |||
| Hfx-2019-SC4-cds7 | narC | - | - | -4.28 | Cytochrome b-561 |
| Hfx-2019-SC4-cds158 | - | - | -2.25 | 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein, N-terminal | |
| Hfx-2019-SC4-cds159 | - | - | - 2.15 | Prokaryotic molybdopterin-containing oxidoreductase family, binding subunit | |
| Hfx-2019-SC4-cds160 | hybA | - | - | -2.89 | Fe-S-cluster-containing hydrogenase components 1 |
| Hfx-2019-SC4-cds161 | - | - | -3.90 | Prokaryotic molybdopterin-containing oxidoreductase family, membrane subunit | |
| Hfx-2019-SC4-cds162 | torD | - | - | -4.79 | Uncharacterized component of anaerobic dehydrogenases |
| Hfx-2019-SC4-cds163 | - | - | -5.16 | Oxidoreductase, molybdopterin-binding domain containing protein | |
| Hfx-2019-SC4-cds242 | - 2.95 | - | - | Thioredoxin (TRX)-like [2Fe-2S] Ferredoxin (Fd) familymember | |
aRNA-Seq was used to determine the expression levels of the genes in Hfx. alexandrinus DSM 27206, as detailed in the section on Materials and Methods. The data are the average of three replicates. The threshold of differential expression genes was: |log2(FoldChange)|> 1 and qvalue < 0.00. Dashes indicate that there were minimal changes to the transcription, not considered statistically significant. bThe gene name, number, and annotation were generated through the gDNA sequencing of the tested strain, as described in the Materials and Methods section