Table 4.
Transcription rates of genes involved cellular motilitya
| Hfx. alexandrinus DSM 27206 ORFb | Gene name | Log2FC | Annotation | ||
|---|---|---|---|---|---|
| 0.1 mM | 0.25 mM | 0.5 mM | |||
| Hfx-2019-SC2-cds599 | flaD/flaE | - | - | -2.02 | Flagellar protein family |
| Hfx-2019-SC2-cds600 | cheD | - | - | -2.03 | Chemotaxis protein |
| Hfx-2019-SC2-cds602 | cheY | - | - | -2.01 | Two-component system chemotaxis family response regulator |
| Hfx-2019-SC2-cds605 | flaB | - | - | -3.39 | Archaeal flagellin |
| Hfx-2019-SC2-cds606 | flaB | - | - | -3.33 | Archaeal flagellin |
| Hfx-2019-SC2-cds616 | cheF | - | - | -2.12 | Chemotaxis protein |
| Hfx-2019-SC3-cds360 | mcp | - | - | -2.32 | Methyl-accepting chemotaxis protein |
aRNA-Seq was used to determine the expression levels of the genes in Hfx. alexandrinus DSM 27206, as detailed in the section on Materials and Methods. The data are the average of three replicates. The threshold of differential expression genes was: |log2(FoldChange)|> 1 and qvalue < 0.00. Dashes indicate that there were minimal changes to the transcription, not considered statistically significant. bThe gene name, number, and annotation were generated through the gDNA sequencing of the tested strain, as described in the Materials and Methods section