Table 5.
Transcription rates of DNA- binding proteinsa
| Hfx. alexandrinus DSM 27206 ORFb | Gene name | Log2FC | Annotation | ||
|---|---|---|---|---|---|
| 0.1 mM | 0.25 mM | 0.5 mM | |||
| Hfx-2019-SC1-cds429 | 2.41 | - | - | Helix-turn-helix domain-containing proteins | |
| Hfx-2019-SC1-cds430 | 3.14 | - | - | Helix-turn-helix domain-containing proteins | |
| Hfx-2019-SC3-cds305 | - | - | 2.09 | Helix-turn-helix domain-containing proteins | |
| Hfx-2019-SC6-cds1 | MC1 | - | - | 2.02 | Non-histone chromosomal protein |
| Hfx-2019-SC1-cds537 | xerD | - | -2.49 | -2.11 | Integrase/recombinase |
| Hfx-2019-SC1-cds560 | sbc | - | -3.3 | - | Exonuclease |
| Hfx-2019-SC1-cds580 | - | - | -2.01 | Orc1-type DNA replication protein | |
| Hfx-2019-SC2-cds231 | - | - | -2.27 | HNH endonuclease | |
| Hfx-2019-SC7-cds13 | - | -4.04 | -4.62 | DNA-binding protein | |
| Hfx-2019-SC7-cds14 | - | -3.56 | -3.95 | HNH endonuclease | |
| Hfx-2019-SC7-cds48 | - | -2.63 | -3.21 | DNA-binding protein | |
aRNA-Seq was used to determine the expression levels of the genes in Hfx. alexandrinus DSM 27206, as detailed in the section on Materials and Methods. The data are the average of three replicates. The threshold of differential expression genes was: |log2(FoldChange)|> 1 and qvalue < 0.00. Dashes indicate that there were minimal changes to the transcription, not considered statistically significant. bThe gene name, number, and annotation were generated through the gDNA sequencing of the tested strain, as described in the Materials and Methods section.