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. 2005 Mar 23;33(6):1767–1778. doi: 10.1093/nar/gki315

Figure 4.

Figure 4

Liquid chromatograms of synthetic 11mers with the sequence 5′-CAGXAATTCGC-3′ (X, hypoxanthine or N1-aminoethyl hypoxanthine). The top panel is the purified 11mer containing hypoxanthine, while the middle panel is the purified 11mer containing N1-(2-aminoethyl)-hypoxanthine. The lower panel is the co-injection of the both 11mers. Conditions: Mono Q column from Pharmacia Biotech; Gradients of eluant A: 0.4 M NaCl and 1 mM NaOH solution (pH 11). Gradients of eluant B: 1.2 M NaCl and 1 mM NaOH solution (pH 11). Programme: eluant B was kept at 0% for first 2 min, and then increased to 15% during the next 3 min, then to 30% during the following 20 min. Flow rate: 1 ml/min. Monitored at 260 nm.