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. Author manuscript; available in PMC: 2024 Jul 1.
Published in final edited form as: Drug Deliv Transl Res. 2022 Dec 24;13(7):1912–1924. doi: 10.1007/s13346-022-01284-6

Figure 2. Pentanoate modulates FoxP3 and IL-17 expression in CD4+ T cells.

Figure 2.

a) Schematic of in vitro cell culture assessing effects of pentanoate concentration on Th17 and Treg differentiation from CD4+ mT cells. b) Representative flow plot of cell viability of CD4+ mT cells in Th17 inducing conditions. (c-d) quantification of c) fold expansion d) % live CD4+ mT cells in Th17 inducing conditions. e) representative flow plot and f) quantification of FoxP3 expression in CD4+ mT cells in inflammatory conditions. (g-i) g) representative flow plot and quantification of h) RORγt and i) IL-17 expression in CD4+ mT cells in the aforementioned culture conditions. Data in c,d,f,h,I are the mean ± S.D. of representative experiments. Statistical analyses in c,d,f,h,I were performed using one-way ANOVA with a post-hoc Dunnet’s multiple comparison test.