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. 2005 Mar 23;33(6):1790–1803. doi: 10.1093/nar/gki319

Figure 2.

Figure 2

Figure 2

Effect of polyamine on uranyl cleavage of kinetoplast DNA. (A) Selected densitometric scans of a titration experiment showing the uranyl cleavage in the absence and presence of 0.2, 0.4, 1.0 and 16 mM spermidine. The DNA sequence is annotated on the scan. Numbers on the top of the scan represent cleavage maxima also shown in (B and C). (B) Uranyl cleavage of both strands of kinetoplast DNA in the absence (white bars) and presence (black bars) of 1 mM spermine. The cleavage intensities were measured by densitometric scanning and are plotted as a function of sequence. (C) Differential cleavage plot comparing the susceptibility of the kinetoplast DNA fragment to uranyl cleavage in the absence and presence of 1 mM spermidine. Black boxes indicate the position of inhibition of uranyl cleavage in the presence of spermidine. Regions with A-tracts are indicated and underlined. (D) Comparison of the effect of putrescine, spermidine and spermine on uranyl cleavage. The effect of polyamine on uranyl cleavage of A-tract number 5 in (A) has been analysed. Uranyl cleavage efficiency without polyamine has been set to 1.0.