FIG. 3.

Real-time PCR analysis of cytokine mRNA expression by DC in response to in vitro FMDV exposure. (A) Kinetic analysis of TNF-α mRNA expression by DC in response to FMDV exposure for two independent experiments. (B) Real-time PCR analysis to confirm the expression of proinflammatory cytokine mRNAs. Whole RNAs were isolated, and mRNA determinations were done by real-time PCR. The analysis was internally controlled by measuring 18S rRNA levels. Cytokine mRNA levels for each treatment were calculated relative to those at 0 h for the kinetic analysis shown in panel A or relative to those in MC at 3 h for the data shown in panel B. Data are expressed as log₁₀ mRNA levels.