FIG. 6.

HeLa cell growth suppression activity of mutated E2 proteins. HeLa cells were transfected with pMEP4 (No E2), wild-type pMEP-E2 (WT E2), or mutated pMEP-E2 constructs as indicated. Transfected cells were selected with 200 μg of hygromycin B/ml and cultured at either 34 or 37°C, as indicated. When the No E2 plate approached confluence, the cells were fixed and stained with methylene blue. Shown are plates from several groups.