TABLE 1.
Mutated amino acids
| Group (description) | E2 Protein | Mutation(s)a |
|---|---|---|
| A (acidic amphipathic alpha-helix) | A1 | E2A, E6A, E13A, E20A |
| A2 | E2Q, E6Q, E13Q, E20Q | |
| B (charge cluster) | B1 | D28N |
| B2 | D24N, K25Q, D28N | |
| B3 | D24A, K25A, D28A | |
| C (replication/E1 interaction) | C1 | R68A |
| C2 | E39A | |
| C3 | E39A, R68A | |
| C4 | Q12A, Q15A, E39A | |
| C5 | Q12A, Q15A, E39A, R68A | |
| C6 | R68Q | |
| C7 | E39Q | |
| C8 | E39Q, R68Q | |
| C9 | Q12N, R68Q | |
| C10 | Q12N, Q15N, E39Q | |
| C11 | Q12N, Q15N, E39Q, R68Q | |
| D (basic cluster) | D1 | R47A, K48A, K49A, R58A, H61A |
| D2 | R47Q, K48Q, K49Q, R58Q, H61N | |
| F (transactivation/self-interaction) | F1 | R37A |
| F2 | I73A | |
| F3 | R37A, I73A | |
| F4 | R37A, K70A, E74A, Q76A, Q80A | |
| F5 | R37A, K70A, I73A, E74A, Q76A, Q80A | |
| F6 | R37Q, K70Q, E74Q, Q76A, Q80A | |
| G (acidic cluster) | G1 | D89A, E90A |
| G2 | E86A, D89A, E90A | |
| G3 | E86Q, D89N, E90Q | |
| H (charge cluster) | H1 | R172A, D175A, E176A |
| H2 | R172A, D175A, E176A, R179A | |
| H3 | R172Q, D175N, E176Q, R179Q | |
| I (charge cluster) | I1 | R189A, D190A, D192A, R193A |
| I2 | R189Q, D190N, D192N, R193Q |
a
Mutations are listed as wild-type E2 amino acid, residue number, mutated amino acid.