TABLE 2.
E2a | No. of cells with:
|
Total no. of cells | % | |||
---|---|---|---|---|---|---|
ON | Both | OFF | No E2 | |||
WT | 38 | 9 | 8 | 3 | 58 | 85 |
A1 | 36 | 5 | 1 | 0 | 42 | 98 |
A2 | 40 | 0 | 0 | 2 | 42 | 100 |
B1 | 19 | 1 | 6 | 6 | 32 | 77 |
B2 | 38 | 1 | 7 | 9 | 55 | 85 |
B3 | 0 | 0 | 47 | 0 | 47 | 0 |
C1 | 35 | 8 | 9 | 2 | 54 | 83 |
C2 | 26 | 14 | 9 | 1 | 50 | 82 |
C3 | 29 | 5 | 16 | 7 | 57 | 68 |
C4 | 2 | 2 | 35 | 5 | 44 | 10 |
C5 | 2 | 0 | 26 | 8 | 36 | 7 |
C6 | 20 | 18 | 6 | 2 | 46 | 86 |
C7 | 27 | 8 | 5 | 0 | 40 | 88 |
C8 | 25 | 9 | 6 | 0 | 40 | 85 |
C9 | 0 | 0 | 30 | 16 | 46 | 0 |
C10 | 0 | 0 | 35 | 0 | 35 | 0 |
C11 | 0 | 0 | 30 | 0 | 30 | 0 |
D1 | 15 | 13 | 14 | 0 | 42 | 67 |
D2 | 16 | 10 | 21 | 0 | 47 | 55 |
F1 | 1 | 15 | 17 | 2 | 35 | 48 |
F2 | 0 | 3 | 31 | 5 | 39 | 9 |
F3 | 0 | 0 | 33 | 7 | 40 | 0 |
F4 | 0 | 0 | 38 | 8 | 46 | 0 |
F5 | 0 | 0 | 30 | 15 | 45 | 0 |
F6 | 0 | 0 | 38 | 6 | 44 | 0 |
G1 | 35 | 10 | 8 | 4 | 57 | 85 |
G2 | 6 | 0 | 0 | 7 | 13 | 100 |
G3 | 35 | 9 | 2 | 1 | 47 | 96 |
H1 | 16 | 9 | 18 | 4 | 47 | 58 |
H2 | 25 | 13 | 11 | 0 | 49 | 78 |
H3 | 18 | 23 | 5 | 2 | 48 | 89 |
I1 | 0 | 0 | 31 | 12 | 43 | 0 |
I2 | 0 | 0 | 36 | 13 | 49 | 0 |
Refer to Table 1 for mutations. WT, wild type.
Mitotic chromosome binding activity is expressed as ability to bind to mitotic chromosomes. ON, exclusive colocalization with mitotic chromosomes; Both, E2 protein localized both on and off mitotic chromosomes; OFF, exclusion from mitotic chromosomes; No E2, no E2 expression; %, percentage of mitotic cells with E2 bound to mitotic chromosome (ON and Both).