TABLE 3.

Summary of data from binding, transactivation, repression, and replication experiments

E2 protein	% Mitotic cells with chromosome binding at:		BRD4 binding in vitro (% of wild type)	Transactivation (% of wild type) ata:		HeLa repression atb:		DNA replicationc	E1 binding in vitroc
	37°C	34°C		37°C	34°C	37°C	34°C
Wild type	85	100	100	100	100	+++	+++	+++	+++
A1	98		79	79	72	+++	+++	±	+
A2	100		78	88	81	+++	+++	±	+
B1	77		74	34	64	±	++	+	+++
B2	85		46	36	59	±	++	++	+++
B3	0	88	15	8	10	±	±	−	+++
C1	83		61	86	106	++	+++	+	+++
C2	82		52	81	93	++	ND	++	+++
C3	68		43	36	68	±	++	++	+++
C4	10	78	23	62	99	+	+++	−	++
C5	7	19	13	18	49	±	++	−	+
C6	86		66	84	102	+++	+++	±	+++
C7	88		53	125	111	+++	+++	++	+++
C8	85		31	50	87	±	++	+	+++
C9	0	36	41	8	15	±	±	−	++
C10	0	0	1	7	7	+	+	−	±
C11	0	0	1	8	6	±	±	−	±
D1	67		84	120	121	+++	+++	±	++
D2	55		76	99	104	+++	+++	+	++
E1	48	46	27	64	61	+	+	+	+++
F2	9	8	21	41	40	+	+	++	+++
F3	0	0	3	30	34	±	±	+++	+++
F4	0	0	1	16	17	±	±	−	+++
F5	0	0	1	19	24	±	±	±	++
F6	0	0	1	10	6	±	±	−	+++
G1	85		58	95	104	++	+++	+	+++
G2	100		40	12	26	+++	+++	±	+++
G3	96		78	103	103	+++	+++	++	+++
H1	58		57	108	127	+	+++	++	+++
H2	78		57	93	119	+	+++	±	++
H3	89		52	80	92	+	+++	±	++
I1	0	25	33	53	79	±	++	±	++
I2	0	67	39	47	76	+	++	±	+++

^aThe E2-responsive luciferase reporter plasmid pBS1073 was cotransfected with the indicated pMEP-E2 constructs. Luciferase expression is expressed relative to wild-type E2. Each mutation was tested in at least three independent transfections.

^bHeLa growth suppression activity, as described in Fig. 6. ND, not done.

^cDNA replication activity and cooperative origin binding activity with the E1 protein are expressed as a percentage of wild-type E2 activity. +++, >60% activity; ++, >30 to 60%; +, >10 to 30% activity; ±, ≤10% activity. Data are from reference 6.