Figure S5.

IL7R signaling regulates CD62L⁺ Tfh cell development. (A) Mean ATAC-seq coverage at the Il7r gene locus in CD62L⁺ Tfh cell, PD1⁺ Tfh, naïve T cell, and in vitro induced Tfh-like cells. (B) Schematic diagram of IL-7R blocking antibody treatment in mice adoptively transferred with naïve OT-II⁺ T cells. (C) Representative flow cytometry plots of CD62L and PD-1 expression in the transferred CD45.2⁺ OT-II⁺ CD4⁺CXCR5⁺Bcl6⁺ Tfh cells from control and IL-7R blocking antibody-treated recipient mice. (D) Statistical analysis of PD-1⁺ and CD62L⁺ cell numbers (upper) and percentages (bottom) in Tfh cells of control and IL-7R blocking antibody-treated recipient mice at indicated time points. (E and F) B6 mice were subcutaneously immunized with KLH and analyzed on day 8 and 21 after immunization. (E) Representative flow cytometry staining of TCF1 versus CD62L and PD-1 in CD4⁺CD44^hiCXCR5⁺Bcl6⁺ cells. (F) TCF1⁺ percentages in PD-1⁺ and CD62L⁺ Tfh cells. (G) GSEA indicated the genes upregulated and downregulated by TCF7 in CD62L⁺ Tfh cells and PD1⁺ Tfh cells. Data of B–F represent two independent experiments. Data are shown as mean ± SD; two-tailed t test; *, P < 0.05; **, P < 0.01; ns, no significance.