TABLE 3.
Effects of increased osmolarity on pHi and glutamate pool levels in the presence of acetatea
| Glucose (mM) | Acetate | Acetate anion (mM)b | μ (h−1) | Inhibition (%) | pHi | Glutamate (mM) |
|---|---|---|---|---|---|---|
| 0 | − | 0.69 ± 0.03 | 7.85 ± 0.05 | 60 ± 6 | ||
| + | 243 ± 28 | 0.34 ± 0.01 | 51 | 7.48 ± 0.05 | 10 ± 2 | |
| 200 | − | 0.53 ± 0.02 | 7.59 ± 0.01 | 117 ± 2 | ||
| + | 161 ± 26 | 0.30 ± 0.04 | 44 | 7.30 ± 0.07 | 48 ± 1 | |
| 400 | − | 0.44 ± 0.03 | 7.61 ± 0.03 | 160 ± 3 | ||
| + | 130 ± 9 | 0.28 ± 0.05 | 36 | 7.21 ± 0.03 | 62 ± 2 |
a
Cells were grown to steady state in the presence or absence of 8 mM acetate; pHi and glutamate pool levels were determined as described in Materials and Methods. The experiments were carried out three times, and the pHi and glutamate pool levels for each experiment were means of three determinations.
b
Acetate pool levels were calculated from a knowledge of the pHi, the external concentrations of acetate anion (7.6 mM), and the pHo of 6.