TABLE 4.
Interdependence of glutamate synthesis and pHi recoverya
| Strain | Acetate (8 mM) | Aspartate (0.03% [wt/vol]) | pHi | Glutamate pool concn (mM) |
|---|---|---|---|---|
| Frag1 | − | − | 7.85 ± 0.05 | 60 ± 6 |
| − | + | 7.88 ± 0.06 | 61 ± 5 | |
| + | − | 7.48 ± 0.05 | 11 ± 2 | |
| −b | − | 7.84 ± 0.04 | 63 ± 6 | |
| Frag83 | − | + | 7.5 ± 0.03 | 50 ± 1.7 |
| + | + | 7.2 ± 0.04 | 12 ± 0.5 | |
| −b | − | 7.2 ± 0.08 | 1.4 ± 0.2 | |
| −b | + | 7.58 ± 0.04 | 59 ± 0.9 |
a
Experiments were performed three times and the data were averaged; five pHi measurements were taken per experiment, and three glutamate pool measurements were taken per experiment.
b
Glutamate pool and pHi after washing of cells to remove acetate and incubation in acetate-free medium for 15 min.