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. 2005 Apr;79(8):4720–4729. doi: 10.1128/JVI.79.8.4720-4729.2005

FIG. 2.

FIG. 2.

Protein profiles of cell and virus lysates of the WT and mutants. The cell and virus lysates for glycine substitution (A and B) and MSD replacement mutants (C and D) were prepared from COS7 transfected with proviral DNA. The Env proteins (gp160, gp120) were detected with anti-gp120 polyclonal antibody. Gag (p24, p17) and Pol (p66, p51) were detected using serum from an individual infected with HIV-1. The name of the provirus DNA is indicated below, and the bands corresponding to gp160, gp120, p66, p51, p24, and p17 are shown by arrows. (C and D) For MSD replacement mutants, two different amounts of lysates were loaded for each mutant. The second lane of each sample received an amount of the lysate that was half of the amount used for the preceding lane.

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