Skip to main content
. Author manuscript; available in PMC: 2024 Jul 6.
Published in final edited form as: Cell Stem Cell. 2023 Jul 6;30(7):950–961.e7. doi: 10.1016/j.stem.2023.06.006

Figure 4. Differential gene expression in POU4F3nT+ cells of D109 ventralized vs. control inner ear organoids.

Figure 4.

(A) FACS gating strategy used to isolate POU4F3nT+ cells from dissociated D109 inner ear organoids in PUR+IWP2 and CTRL conditions.

(B-C) UMAP plot of POU4F3nT+ cells isolated from D109 PUR+IWP2 and CTRL inner ear organoids (B) and UMAP plot grouped by experimental conditions (C). The number of sequenced cells: 7,968 (CTRL), 11,107 (PUR+IWP2). The number of cells after QC filtering: 5,971 (CTRL), 10,073 (PUR-IWP2).

(D) Heat map showing gene expression across clusters.

(E) Feature plots showing the distributions of marker genes across the cluster map and those showing differential expression of known cochlear and vestibular marker genes in annotated hair cell populations.

DRG; dorsal root ganglion, FP; floor plate, NC; neural crest.