TABLE 1.
Summary of Ebola virus GP1 substitution mutants
| GP1 | Protein expression | Viral incorporation | Infectivity (%) | Classa | Note |
|---|---|---|---|---|---|
| wt | ++++ | ++++ | 100 | Full length sequenced | |
| M1 (D47A/D49A/K50A) | ++++ | ++++ | 17 | IV | Full length sequenced |
| M2 (R54A/D55A/K56A) | ++++ | +++ | 1 | III | Full length sequenced |
| M6 (K84A/R85A) | + | + | 0 | II | Full length sequenced |
| M10 (E103A/E106A) | + | + | 0 | II | Full length sequenced |
| M11 (E112A/K114A/K115A) | ++++ | +++ | 10 | IV | Full length sequenced |
| M12 (D117A/E120A) | +++ | ++ | 17 | III | Full length sequenced |
| M13 (D127A/R130A) | ++++ | ++++ | 71 | I | |
| M14 (R134A/R136A) | ++++ | ++ | 59 | III | |
| M15 (H139A/K140A) | + | + | 1 | II | Full length sequenced |
| M17 (H154A/K155A) | + | + | 1 | II | Full length sequenced |
| M18 (D163A/R164A) | ++++ | +++ | 58 | I | |
| M21 (K190A/K191A) | ++++ | ++++ | 44 | I | |
| M22 (R200A/E201A) | ++++ | ++++ | 77 | I | |
| M23 (E207A/D208A) | ++++ | ++++ | 75 | I | |
| M25 (E235A/D237A) | ++++ | ++++ | 54 | I | |
| L43A | ++++ | +++ | 45 | III | |
| D47A | ++++ | ++++ | 53 | I | |
| D49A | ++++ | ++++ | 55 | I | |
| K50A | ++++ | ++++ | 89 | I | |
| L51A | ++++ | +++ | 21 | III | |
| R54A | ++++ | ++++ | 50 | I | |
| D55A | ++++ | ++++ | 13 | IV | Full length sequenced |
| D55E | ++++ | ++++ | 111 | I | |
| D55K | ++++ | ++++ | 89 | I | |
| K56A | ++++ | ++++ | 46 | I | |
| L57A | ++++ | +++ | 2 | IV | Full length sequenced |
| L57I | ++++ | ++++ | 7 | IV | |
| L57F | ++++ | ++++ | 5 | IV | |
| L57K | ++++ | ++++ | 5 | IV | |
| Q62A | ++++ | ++++ | 195 | I | |
| L63A | ++++ | ++++ | 14 | IV | |
| L63I | ++++ | ++++ | 78 | I | |
| L63F | +++ | ++ | 2 | II | |
| L63K | ++ | + | 0 | II | |
| M3 (R64A) | ++++ | ++++ | 0 | IV | Full length sequenced |
| R64K | ++++ | ++++ | 120 | I | |
| R64E | ++++ | +++ | 0 | IV | |
| L68A | ++ | +++ | 3 | II | |
| N69A | ++++ | ++++ | 98 | I | |
| L70A | ++++ | ++++ | 95 | I | |
| M4 (E74A) | +++ | + | 2 | III | Full length sequenced |
| M5 (D78A) | ++++ | ++++ | 115 | I | |
| K84A | ++++ | ++++ | 94 | I | |
| R85A | + | + | 0 | II | Full length sequenced |
| W86A | + | ++ | 8 | II | |
| F88A | ++++ | ++++ | 1 | IV | Full length sequenced |
| F88I | ++++ | ++++ | 104 | I | |
| F88E | ++++ | ++ | 0 | III | |
| M7 (R89A) | +++ | +++ | 59 | I | |
| M8 (K95A) | ++++ | ++++ | 8 | IV | Full length sequenced, second mutation V48I |
| K95R | ++++ | +++ | 74 | I | |
| K95E | ++++ | ++ | 26 | III | |
| Y99A | ++ | + | 3 | II | |
| M9 (E100A) | ++++ | ++++ | 48 | I | |
| E103A | + | + | 0 | II | Full length sequenced |
| W104A | ++++ | ++++ | 92 | I | |
| E106A | ++++ | ++++ | 87 | I | |
| N107A | ++++ | ++++ | 79 | I | |
| Y109A | + | + | 9 | II | |
| N110A | ++++ | ++++ | 183 | I | |
| L111A | ++++ | + | 2 | III | |
| E112A | ++++ | ++++ | 67 | I | |
| I113A | ++++ | ++ | 25 | III | |
| K114A | ++++ | ++++ | 53 | I | |
| K115A | ++++ | ++++ | 56 | I | |
| D117A | +++ | + | 17 | III | |
| E120A | ++++ | ++ | 35 | III | |
| L122A | ++++ | ++ | 4 | III | Full length sequenced |
| F132A | ++++ | + | 35 | III | |
| Y137A | ++++ | + | 75 | III | Full length sequenced |
| H139A | + | + | 2 | II | Full length sequenced |
| K140A | ++++ | ++++ | 74 | I | |
| M16 (D150A) | ++++ | ++++ | 135 | I | |
| F153A | ++++ | ++ | 31 | III | |
| H154A | + | + | 0 | II | Full length sequenced |
| K155A | ++++ | ++++ | 61 | I | |
| F159A | ++ | ++ | 1 | II | |
| L161A | ++ | + | 1 | II | |
| Y162A | ++ | + | 2 | II | |
| L165A | ++++ | ++++ | 55 | I | |
| I170A | ++++ | ++++ | 16 | IV | Full length sequenced |
| I170F | ++++ | + | 45 | III | |
| I170E | ++++ | + | 0 | III | |
| Y171A | + | ++ | 1 | II | |
| M19 (R172A) | ++++ | ++++ | 55 | I | |
| F176A | + | + | 0 | II | |
| M20 (E178A) | + | + | 0 | II | Full length sequenced |
| F183A | ++ | + | 0 | II | |
| L184A | ++++ | +++ | 27 | III | |
| I185A | ++++ | +++ | 23 | III | Full length sequenced |
| L186A | ++++ | ++++ | 66 | I | |
| F193A | ++++ | ++++ | 79 | I | |
| F194A | ++++ | ++++ | 67 | I | |
| N204A | ++++ | ++++ | 74 | I | |
| G212A | ++++ | ++++ | 73 | I | |
| Y213A | ++++ | ++++ | 75 | I | |
| Y214A | ++++ | ++++ | 69 | I | |
| I218A | +++ | + | 43 | III | |
| Y220A | +++ | + | 32 | III | |
| F225A | +++ | + | 58 | III | |
| M24 (E229A) | ++++ | ++++ | 61 | I | |
| Y232A | ++++ | ++++ | 82 | I | |
| L233A | ++++ | ++++ | 82 | I | |
| F234A | ++++ | ++++ | 98 | I | |
| Y241A | ++++ | ++++ | 81 | I | |
| L244A | ++++ | ++++ | 72 | I | |
| M26 (E245A) | ++++ | ++++ | 55 | I | |
| F248A | ++++ | ++++ | 105 | I | |
| Q251A | ++++ | ++++ | 83 | I | |
| F252A | ++++ | ++++ | 74 | I | |
| L253A | ++++ | ++++ | 85 | I | |
| Q255A | ++++ | ++++ | 101 | I | |
| L256A | ++++ | ++++ | 99 | I | |
| N257A | ++++ | ++++ | 83 | I | |
| M27 (E258A) | ++++ | ++++ | 101 | I | |
| I260A | ++++ | ++++ | 110 | I | |
| Y261A | ++++ | ++++ | 111 | I | |
| N268A | ++++ | ++++ | 91 | I | |
| L273A | ++++ | ++++ | 85 | I | |
| W275A | ++++ | ++++ | 109 | I |
a
Class I, no major defect in protein expression or processing, virion incorporation, or viral entry (wild-type phenotype); class II, defect in protein expression or processing; class III, defect in virion incorporation; class IV, defect in viral entry.